Supplementary material


Photochemical Micromachining of Lysozyme Crystals

O. D. Velev, E. W. Kaler and A. M. Lenhoff

Adv. Mater., 11, 1345 (1999).


 

Fluorescence spectra

Emission and excitation fluorescence spectra of Pyr-C3-COOH in the absence of lysozyme, after introducing the protein and after photobleaching. The spectra demonstrate the slow but strong quenching effect of the protein and the partial fluorescence recovery after bleaching.

 
 

     Light micromachnining schematics

 
Schematic of the concept for crystal micromachining via photochemical denaturation (original color figure).

 
 

Drilled crystal Drilling scheme      

 
Evidence of the effect of the converging and diverging light beams on the micromachining of lysozyme crystals. The crystal in the micrograph has been treated photochemically and then flipped on one of its sides to show the internal structure. The microscope had been focussed on the top surface of the crystal (to the left in the Figure). The light beam has been converging above the surface and diverging below it. The crystal has a hole where the protein has been denatured photochemically and then dispersed in a diverging beam. On top of the hole, however, there is a black blob of protein deposited by the converging light beam. Thus, while the protein in the diverging light beam has been removed, additional protein has been deposited in the converging beam above the surface. Scale bar = 50 mm.

 

 

 

Reference:   O. D. Velev, E. W. Kaler and A. M. Lenhoff, Adv. Mater., 11, 1345 (1999).

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Posted on 12/27/99