Assembly of Protein Structures onto Liposomes by Using Non-specific and Specific Interactions
Three distinct interactions were used for this assembly:
Bar = 100 nm.
Ferritin structures assembled by electrostatic interaction and cross-linking. TEM of negatively stained samples.
a) Vesicle with adherent non-fixed ferritin array. The liposomes are positively charged by incorporating some amount of HTAB.
b) Ferritin shell cross-linked by glutaraldehyde over a liposome;
c) Ferritin aggregate obtained after the liposomes are dissolved by solubilisation with non-ionic surfactant (Tween 20).
Bar = 100 nm.Liposome/ferritin assemblies obtained by "lock-and-key" binding.
Complex microstructured particles could be assembled from liposomes and ferritin by using colloid interaction schemes including either non-specific or a combination between non-specific and specific interactions. The acquired data can find application in the future fabrication of microstructured, multicomponent or functionalised protein and liposome/protein assemblies. The knowledge, control, and multi-step modification of the colloid interactions in the system appear to be the real tool for "smart" particle assembly.
O. D. Velev, Adv. Biophys., 34, 139 (1997).
© Copyright O. D. Velev 1998